Comparison of conventional and shifted excitation Raman difference spectroscopy for bacterial identification

Raman spectroscopy is an emerging tool for fast bacterial identification. However, depending on suitable preprocessing of the spectra, thereby background removal a decisive step conventional spectroscopy. The has to be estimated, which challenging especially high fluorescence backgrounds. Shifted excitation difference (SERDS) eliminates through experimental procedure and holds as promising approach highly fluorescent samples. Bacterial spectra might complex because these consist multitude overlapping bands from large multiplicity biomolecules only subtitle differences between species enable Here, we investigate benefits SERDS compared with specific study identification bacteria. comparison utilizes sets four measured covers three processing approaches example, reconstruction non-negative least square algorithm.